Exploring a broad spectrum nitrilase from moderately halophilic bacterium Halomonas sp. IIIMB2797 isolated from saline lake. (25th July 2018)

• Record Type: Journal Article
• Authors: Singh, Rahul Vikram; Sharma, Hitesh; Koul, Anshela; Babu, Vikash
• Is Part Of: Journal of basic microbiology. Volume 58:issue 10(2018)
• Page Start: 867
• Page End: 874
• Publication Date: 2018-07-25
• Subjects: biotransformation -- Halomonas -- moderate halophile -- nitrilase; Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201800168
• Languages: English
• ISSNs: 0233-111X

Abstract

Abstract : Nitrile hydrolyzing moderate halophilic bacterium Halomonas sp. IIIMB2797 was isolated from Sambhar Lake, India. Maximum cell biomass and nitrilase production were observed at 60 g L −1 NaCl in the production media which confirms its moderate halophilic nature. Nitrilase of Halomonas sp. IIIMB2797 proved to be inducible in nature as maximum activity was observed when valeronitrile was added in the production media. Whole cells of Halomonas sp. IIIMB2797 exhibited broad substrate affinity towards aromatic and aliphatic nitriles. Optimum pH and temperature for nitrilase activity was observed at 7.0 and 45 °C, respectively. Effect of salinity on nitrilase activity was also studied and maximum activity was observed in presence of 50 g L −1 NaCl in 0.1 M phosphate buffer of pH 7.0. The interesting feature of the study is that whole cells of Halomonas sp. IIIMB2797 exhibited higher nitrilase activities in presence of organic solvents which may be useful in biotransformation of nitriles to corresponding carboxylic acids for industrial applications.