Hippocampal amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid GluA1 (AMPA GluA1) receptor subunit involves in learning and memory improvement following treatment with Centella asiatica extract in adolescent rats. Issue 9 (14th August 2018)

• Record Type: Journal Article
• Authors: Binti Mohd Yusuf Yeo, Nor Aqilah; Muthuraju, Sangu; Wong, Jia Hui; Mohammed, Faruque Reza; Senik, Mohd Harizal; Zhang, Jingli; Yusof, Siti Rafidah; Jaafar, Hasnan; Adenan, Mohd llham; Mohamad, Habsah; Tengku Muhammad, Tengku Sifzizul; Abdullah, Jafri Malin
• Is Part Of: Brain and behavior. Volume 8:Issue 9(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-08-14
• Subjects: adolescent -- AMPA receptor -- Centella asiatica -- GABA receptor -- hippocampus -- learning and memory; Neurology -- Periodicals; Neurosciences -- Periodicals; Psychology -- Periodicals; Psychiatry -- Periodicals; 616.8005
• DOI: 10.1002/brb3.1093
• Languages: English
• ISSNs: 2162-3279

Abstract

Abstract: Introduction: Centella asiatica is an herbal plant that contains phytochemicals that are widely believed to have positive effects on cognitive function. The adolescent stage is a critical development period for the maturation of brain processes that encompass changes in physical and psychological systems. However, the effect of C. asiatica has not been extensively studied in adolescents. The aim of this study was therefore to investigate the effects of a C. asiatica extract on the enhancement of learning and memory in adolescent rats. Methods: The locomotor activity, learning, and memory were assessed by using open field test and water T‐maze test. This study also examined changes in neuronal cell morphology using cresyl violet and apoptosis staining. We also performed immunohistochemical study to analyse the expression of the glutamate AMPA receptor (α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid) GluA1 subunit and the GABA receptor (γ‐Aminobutyric Acid) subtype GABAA α1 subunit in the hippocampus of the same animals. Results: We found no significant changes in locomotor activity ( p > 0.05). The water T‐maze data showed that 30 mg/kg dose significantly ( p < 0.05) improved learning, memory, and the memory consolidation phase but had no effect on reversal learning ( p > 0.05). Histological data revealed no neuronal morphological changes. Immunohistochemical analysis revealed increased expression of the AMPA GluA1 receptor subunit but there was no effect on GABAA receptor α1 subunit expression in the CA1 and CA2 subregions of the hippocampus. Conclusions: The C. asiatica extract therefore improved hippocampus‐dependent spatial learning and memory in a dose‐dependent manner in rats through the GluA1‐containing AMPA receptor in the CA1 and CA2 sub regions of the hippocampus. Abstract : The effect of Centella asiatica has been evaluated in adult animals until today. This work is investigated centell asiatica in adolescent rats also analysis expression of AMPA and GABA receptors in hippocampus in rats. Adolescent age is considered to be critical developmental stage of animals as well as humans.