Investigation of Detergent‐Modified Enzymomimetic Activities of TEMED‐Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake. Issue 36 (25th September 2018)

• Record Type: Journal Article
• Authors: Peskova, Marie; Heger, Zbynek; Dostalova, Simona; Fojtu, Michaela; Castkova, Klara; Ilkovics, Ladislav; Vykoukal, Vit; Pekarik, Vladimir
• Is Part Of: ChemistrySelect. Volume 3:Issue 36(2018)
• Page Start: 10139
• Page End: 10146
• Publication Date: 2018-09-25
• Subjects: Detergents -- fluorescent detection -- haloperoxidase, nanoceria hydrate -- TEMED; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201802496
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Ceria nanoparticles can catalyze many redox reactions that resemble reactions catalyzed by naturally occurring enzymes, such as peroxidase, catalase, haloperoxidase or phosphatase. This makes nanoceria an attractive material for biomedical and sensing applications. Presence of detergents dramatically affects the catalytic activity of nanoceria. The catalytic activity is also strongly influenced by biogenic compounds present in the sample. Furthermore, biomedical applications are hindered by the difficulties to detect nanoceria uptake by cells. Therefore, we have developed a new fast and sensitive fluorescent haloperoxidase assay based on fluorescein bromination in presence of CTAB to detect uptake of CeO2 nanoparticles by cells in vitro . Moreover, catalytic activities of standard nanoceria (CeO2 ) were compared to nanoceria hydrates (CeO2 .xH2 O) that are easier for preparation and might be stabilized by biologically relevant molecules allowing for a specific targeting to cells or tissues. We have developed a new approach to produce CeO2 .xH2 O in presence of TEMED and analyzed whether the presence of H2 O2 during the synthesis influences the catalytic activity of produced particles. Abstract : Ceria hydrate NPs are produced from common precursor in presence of TEMED. Synthesized NPs possess, among others, haloperoxidase activity that is blocked by biogenic phosphates but can be restored by cationic detergents and used for detection of ceria in fixed cells.ABTS, 2, 2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid); AOT, dioctyl sulfosuccinate sodium salt; BZ, benzalkonium chloride; CTAB, cetyltrimethylammonium bromide; CB, Celestine blue; DOC, sodium deoxycholate; EO, eosin Y; FCS, fetal calf serum; FL, fluorescein; NC, nanoceria; NCH, nanoceria hydrate; NP, nanoparticle; PBS, phosphate buffered saline; pNPP, p‐nitrophenol phosphate; PR, phenol red; PXRD, powder X‐ray diffraction; SDS, sodium dodecylsulfate; SOD, superoxide dismutase; TB, thymol blue; TBAB, tetrabutyl ammonium bromide; TEA, triethanol amine; TEMED, tetramethylenediamine; TMB, tetramethyl benzidine; Tw20, Tween 20; TX100, Triton X‐100.