Comparison of Tris egg yolk‐based, Triladyl® and Optixell® extender on post‐thaw quality, Kinematics and in vivo fertility of Nili Ravi Buffalo (Bubalus bubalis) bull spermatozoa. (19th June 2018)

• Record Type: Journal Article
• Authors: Naz, Sadia; Umair, Muhammad; Iqbal, Sajid
• Is Part Of: Andrologia. Volume 50:Number 8(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-06-19
• Subjects: Andrology -- Periodicals; Infertility, Male -- Periodicals; 616.69
• DOI: 10.1111/and.13063
• Languages: English
• ISSNs: 0303-4569

Abstract

Abstract: Our objectives were to ascertain the comparison of Tris egg yolk‐based, Triladyl ® and Optixell ® extender on postthaw quality, CASA parameters and in vivo fertility of Nili Ravi buffalo ( Bubalus bubalis ) bull spermatozoa. Semen samples ( n = 35) from five bulls were diluted in Tris egg yolk‐based, Triladyl ®, Optixell ® extender and frozen in 0.50 ml French straws. Postthaw sperm CASA motility (%) was higher ( p < 0.05) in Optixell extender as compared to Triladyl and Tris egg yolk‐based extender. Although sperm progressive motility (%), morphology (%), average path velocity (μm/s), straight line velocity (μm/s), curvilinear velocity (μm/s), amplitude of lateral head displacement (μm), beat cross‐frequency (Hz), straightness (%), length of curvilinear path (μm), length of average path (μm), intact plasma and acrosome membrane (%), and DNA integrity (%) were higher ( p < 0.05) in spermatozoa cryopreserved in Optixell ® extender as compared to Tris egg yolk‐based and Triladyl ® extender. The fertility rates (68.18%, 45.45%, 55.4%) were higher ( p < 0.05) in buffaloes inseminated with semen doses frozen in Optixell extender than the Tris egg yolk‐based and Triladyl ® extender respectively. It is concluded that Optixell ® extender improves postthaw semen quality and fertility in Nili Ravi buffaloes.