Intravoxel incoherent motion MRI for monitoring the therapeutic response of hepatocellular carcinoma to sorafenib treatment in mouse xenograft tumor models. (September 2017)

• Record Type: Journal Article
• Authors: Lee, Yedaun; Lee, Seung Soo; Cheong, Hyunhee; Lee, Chang Kyung; Kim, Namkug; Son, Woo-Chan; Hong, Seung Mo
• Is Part Of: Acta radiologica. Volume 58:Number 9(2017:Sep.)
• Page Start: 1045
• Page End: 1053
• Publication Date: 2017-09
• Subjects: Intra-voxel incoherent motion (IVIM) -- monitoring treatment response -- sorafenib -- hepatocellular carcinoma -- mouse xenograft model; Radiology, Medical -- Periodicals; Radiography, Medical -- Periodicals; Radiotherapy -- Periodicals; 616.0757
• DOI: 10.1177/0284185116683576
• Languages: English
• ISSNs: 0284-1851

Abstract

Background: With the introduction of targeted therapies, there has been a growing need for non-invasive imaging methods which accurately evaluate therapeutic effects and overcome the limitations of tumor size-based therapeutic response assessments. Purpose: To assess diagnostic values of intra-voxel incoherent motion (IVIM) imaging in evaluating therapeutic effects of sorafenib on hepatocellular carcinoma (HCC) using mouse xenograft model. Material and Methods: Twenty-four mice bearing Huh-7 were divided into a control group and two treatment groups received sorafenib doses of 5 mg/kg (5 mg-Tx) or 30 mg/kg (30 mg-Tx). IVIM imaging was performed using 10 b-values (0–900 s/mm 2 ). The apparent diffusion coefficient (ADC), diffusion coefficient ( D ), and perfusion fraction ( f ) were measured for whole tumors and tumor periphery. Changes between baseline and post-treatment parameters ( Δ ADC, Δ D, and Δ f ) were calculated, and these parameters were compared with microvessel density (MVD) and area of tumor cell death. Results: The post-treatment f and Δ f for tumor periphery were significantly higher in control group, followed by 5 mg-Tx and 30 mg-Tx ( P < 0.001). MVD showed significant positive correlation with post-treatment f ( r = 0.584, P = 0.003) and negative correlation with D ( r = –0.495, P = 0.014) for tumor periphery, while no parameter showed significant correlation with area of tumor cell death. Conclusion: The f is significantly correlated with MVD of HCC, and could potentially be used to evaluate the anti-angiogenic effects of sorafenib.