Study of dynamic of chicken infectious anaemia virus infection: which sample is more reliable for viral detection?. Issue 5 (3rd September 2018)

• Record Type: Journal Article
• Authors: Vagnozzi, Ariel E.; Espinosa, Rodrigo; Cheng, Sunny; Brinson, Denise; O'Kane, Peter; Wilson, Jeanna; Zavala, Guillermo
• Is Part Of: Avian pathology. Volume 47:Issue 5(2018)
• Page Start: 489
• Page End: 496
• Publication Date: 2018-09-03
• Subjects: Chicken infectious anaemia virus -- CIAV -- virus detection -- infection dynamic -- real time PCR -- viral load; Poultry -- Diseases -- Periodicals; Birds -- Diseases -- Periodicals; 571.918
• DOI: 10.1080/03079457.2018.1492089
• Languages: English
• ISSNs: 0307-9457

Abstract

ABSTRACT: Chicken infectious anaemia virus (CIAV) is a widely distributed immunosuppressive agent. SPF flocks and eggs used for vaccine production and diagnostics must be CIAV-free. Detection of CIAV infection in SPF flocks involves primarily serology or other invasive methods. In order to evaluate different types of samples for rapid detection of CIAV infection, a trial was conducted in serologically negative broiler breeder pullets vaccinated with a commercial live-attenuated CIAV vaccine. Controls and vaccinated groups were sampled before and after vaccination. Invasive and non-invasive samples were used for CIAV DNA detection by real-time PCR. Seroconversion occurred at 14 days post-inoculation (DPI) in the vaccinated group, whereas CIAV genome was detected by qPCR at 7 DPI in both invasive and non-invasive samples. Only invasive samples remained qPCR positive for CIAV DNA by 21 DPI despite seroconversion of the chickens.