Selective and sensitive detection of cysteine in water and live cells using a coumarin–Cu2+ fluorescent ensemble. (4th September 2018)

• Record Type: Journal Article
• Authors: Wang, Yue; Meng, Qingtao; Han, Qian; He, Guangjie; Hu, Yaoyun; Feng, Huan; Jia, Hongmin; Zhang, Run; Zhang, Zhiqiang
• Is Part Of: New journal of chemistry. Volume 42:Number 19(2018)
• Page Start: 15839
• Page End: 15846
• Publication Date: 2018-09-04
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c8nj03809k
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A coumarin–Cu 2+ ensemble based fluorescent chemosensor was developed for the selective detection of cysteine in aqueous media and live cells. Abstract : Effective discrimination of cysteine (Cys) from biothiols and other amino acids has gained increasing attention due to the essential roles of this molecule in biological systems. In this work, we report a new strategy for the detection of Cys in aqueous media and live cells using a simple Cu 2+ ensemble (L –Cu 2+ ). A coumarin–salicylaldehyde hydrazone fluorescent ligand (L ) was initially synthesized and well characterized.L features high affinity towards Cu 2+ ( K a = 1.995 × 10 6 M −1 ), accompanied by effective quenching of fluorescence in HEPES buffer. The in situ generated chemosensing ensemble, L –Cu 2+, is able to specifically respond to Cys over other biothiols and amino acids through a displacement approach. As a result, a remarkable recovery of fluorescence and UV-vis absorption spectra can be observed.L –Cu 2+ showed high selectivity and sensitivity towards Cys. The detection limit is estimated to be 15 nM. UsingL –Cu 2+ as the fluorescent probe, imaging of Cys in live A549 cells was successfully demonstrated.