A cobalt-based polyoxometalate nanozyme with high peroxidase-mimicking activity at neutral pH for one-pot colorimetric analysis of glucose. Issue 36 (24th August 2018)

• Record Type: Journal Article
• Authors: He, Yanfang; Li, Xin; Xu, Xuechao; Pan, Jianming; Niu, Xiangheng
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 36(2018)
• Page Start: 5750
• Page End: 5755
• Publication Date: 2018-08-24
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb01853g
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A polyoxometalate (CoPW11 O39 ) with high peroxidase-mimicking activity at physiological pH enables one-pot colorimetric analysis of glucose when coupled with GOx. Abstract : Thanks to the attractive merits of excellent robustness, easy large-scale production, and low cost, peroxidase-mimicking nanomaterials (nanozymes) are being used as promising alternatives to horseradish peroxidase in various applications. However, most of the currently developed peroxidase nanozymes only work in acidic media, which seriously hinders their use in biological systems. In this contribution, we reported a new cobalt-based polyoxometalate (CoPW11 O39 ) with high peroxidase-like activity at neutral pH. With the negatively charged surface, the nanozyme exhibited strong ability to catalyze the oxidation of colorless 3, 3′, 5, 5′-tetramethylbenzidine into its blue product mediated by H2 O2 under physiological conditions. Considering that the CoPW11 O39 nanozyme and glucose oxidase (GOx) have a similar pH for catalytic activities, a one-pot colorimetric assay based on the combination of GOx and the peroxidase nanozyme was developed for glucose detection. Our assay has advantages of easy operation and fast detection, providing excellent accuracy for the monitoring of glucose in human plasma. These unique characteristics endow the polyoxometalate nanozyme with bright prospects in biochemical analysis.