A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions. (June 2015)

• Record Type: Journal Article
• Authors: Wang, Li-Chao; Zhang, Wen-Song; Liu, Qun; Li, Jia; Alolga, Raphael N.; Liu, Kang; Liu, Bao-Lin; Li, Ping; Qi, Lian-Wen
• Is Part Of: Journal of functional foods. Volume 16(2015)
• Page Start: 20
• Page End: 27
• Publication Date: 2015-06
• Subjects: Acute myocardial infarction -- Apoptosis -- Cardioprotection -- Endoplasmic reticulum stress -- Rare ginsenoside-standardized extract -- Steamed Panax notoginseng; Notoginsenoside R1 (PubChem CID: 441934) -- Ginsenoside Rb1 (PubChem CID: 9898279) -- Ginsenoside Rg1 (PubChem CID: 441923) -- 20(S)-Ginsenoside Rg3 (PubChem CID: 9918693) -- 20(R)-Ginsenoside Rg3 (PubChem CID: 46887680) -- Ginsenoside Rg5 (PubChem CID: 6450175) -- Ginsenoside Rh4, (PubChem CID: 21599928) -- BAPTA-AM (PubChem CID: 2293) -- Diltiazem hydrochloride (PubChem CID: 62920) -- Tauroursodeoxycholic acid (PubChem CID: 9848818); AMI acute myocardial infarction -- +dP/dtmax maximal rate of pressure development for contraction -- −dP/dtmax maximal rate of pressure development for relaxation -- ER endoplasmic reticulum -- LAD left anterior descending -- LVEDP left ventricular end-diastolic pressure -- LVSP left ventricular systolic pressure -- NG notoginsenoside -- OGD oxygen and glucose deprivation -- RGSE rare ginsenoside-standardized extract -- TUDCA tauroursodeoxycholic acid; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.04.018
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: RGSE attenuated acute myocardial infarction-induced heart injury. RGSE inhibited oxidative stress and apoptosis during AMI in vivo and in vitro . TXNIP-NLRP3-IL-1β signaling, for the first time, was revealed to be activated in AMI, promoting cell death in the end. RGSE exerted cardioprotection by suppression of TXNIP/NLRP3 activation during ER stress. Ginsenosides Rh4, Rg5, Rk3, and Rk1 were the most abundant, and notoginsenoside T5/isomer was first identified in steamed notoginseng. Abstract: Panax notoginseng possesses therapeutic potential for cardiovascular disorders. Endoplasmic reticulum (ER) stress plays a critical role in cardiovascular disease. Here we show that a rare ginsenoside-standardized extract (RGSE) from notoginseng exerts cardioprotection via attenuating ER stress-associated apoptosis. A total of 18 ginsenosides with a concentration of 816.75 mg/g were characterized and quantified in RGSE. Ginsenoside Rh4, Rg5, Rk3, and Rk1 were the most abundant. RGSE at 50 and 100 mg/kg significantly attenuated myocardial infarction and improved cardiac function in acute myocardial infarction (AMI). RGSE also prevented myocardial ischemia-induced oxidative stress and apoptosis in vivo and in vitro . In addition, RGSE decreased the overexpression of GRP78, GRP94 and CHOP, and inhibited the phosphorylation of PERK and IRE1α in ER stress. Further investigation revealed that RGSE effectually inactivated TXNIP/NLRP3 signaling pathway in AMI hearts. These observations suggest a novel role for notoginseng in regulating AMI.