Biobutanol production by a new aerotolerant strain of Clostridium acetobutylicum YM1 under aerobic conditions. (15th October 2015)

• Record Type: Journal Article
• Authors: Al-Shorgani, Najeeb Kaid Nasser; Kalil, Mohd Sahaid; Yusoff, Wan Mohtar Wan; Hamid, Aidil Abdul
• Is Part Of: Fuel. Volume 158(2015)
• Page Start: 855
• Page End: 863
• Publication Date: 2015-10-15
• Subjects: ABE acetone–butanol–ethanol -- DO dissolved oxygen -- OD600 optical density at 600 nm -- DTT dithiothreitol -- DTE dithioerythritol -- GC-FID gas chromatography equipped with a flame ionization detector -- NADH oxidase nicotinamide adenine dinucleotide-oxidase -- NADPH oxidase nicotinamide adenine dinucleotide phosphate-oxidase -- SOD superoxide dismutase -- TYA tryptone yeast-extract acetate medium; Aerobic fermentation -- Clostridium acetobutylicum YM1 -- Biobutanol -- Batch fermentation; Fuel -- Periodicals; Coal -- Periodicals; Coal; Fuel; Periodicals; 662.6
• DOI: 10.1016/j.fuel.2015.05.073
• Languages: English
• ISSNs: 0016-2361

Abstract

Highlights: Introducing a new aerotolerant strain of Clostridium acetobutylicum YM1. Strain YM1 is capable of producing 12.18 g/L biobutanol under aerobic conditions from 50 g/L glucose. Addition of reducing chemicals improved the production of biobutanol under aerobic conditions. Supplementation the culture with 0.01 g/L potassium ferricyanide resulted in 12.94 g/L biobutanol. YM1 was found to be able to produce catalase, SOD and NADH/NADPH oxidases. Abstract: A newly isolated strain of Clostridium acetobutylicum YM1 has a unique property of producing biobutanol from glucose under aerobic conditions. This strain exhibited the capability to grow and produce high concentrations of biobutanol under different concentrations of dissolved oxygen (DO). The growth profile and solvent production in a 5 L bioreactor were similar under anaerobic and aerobic conditions (100% initial DO saturation), and the final biobutanol production was 12.18 and 12.30 g/L, respectively. The addition of reducing chemical agents, without creating anaerobic conditions, to the culture of YM1 enhanced the production of biobutanol. Strain YM1 possesses different enzymes that are responsible for oxygen scavenging, such as superoxide dismutase (SOD), catalase and NADH/NADPH oxidases. This study provides a simple operation strategy for more efficient biobutanol production by using an aerotolerant strain of C. acetobutylicum YM1 without the need to flush the medium with nitrogen gas to ensure anaerobic conditions.