Interactions of multiwalled carbon nanotubes with algal cells: Quantification of association, visualization of uptake, and measurement of alterations in the composition of cells. (January 2015)

• Record Type: Journal Article
• Authors: Rhiem, Stefan; Riding, Matthew J.; Baumgartner, Werner; Martin, Francis L.; Semple, Kirk T.; Jones, Kevin C.; Schäffer, Andreas; Maes, Hanna M.
• Is Part Of: Environmental pollution. Volume 196(2015)
• Page Start: 431
• Page End: 439
• Publication Date: 2015-01
• Subjects: Carbon nanotubes -- Green algae -- Bioavailability -- Electron microscopy -- FTIR spectroscopy -- Nanoparticles; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2014.11.011
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Carbon nanotubes (CNTs) are considered promising materials in nanotechnology. We quantified CNT accumulation by the alga Desmodesmus subspicatus . Cells were exposed to radiolabeled CNTs ( 14 C-CNTs; 1 mg/L) to determine uptake and association, as well as elimination and dissociation in clear media. Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was used to detect effects of CNTs on algae. CNT-cell interactions were visualized by electron microscopy and related to alterations in their cell composition. A concentration factor of 5000 L/kg dry weight was calculated. Most of the material agglomerated around the cells, but single tubes were detected in the cytoplasm. Computational analyses of the ATR-FTIR data showed that CNT treated algae differed from controls at all sampling times. CNT exposure changed the biochemical composition of cells. The fact that CNTs are bioavailable for algae and that they influence the cell composition is important with regard to environmental risk assessment of this nanomaterial. Highlights: Carbon nanotubes were shown to be bioavailable for green algae. Accumulation and elimination was measured using radiolabeled CNT. The nanomaterial was shown to alter the composition of the algal cells. Abstract : Carbon nanotubes were found to be bioavailable for algae and to influence their cell composition, which are important findings with regard to their environmental risk assessment.