A novel Tc-99 m and fluorescence labeled peptide as a multimodal imaging agent for targeting angiogenesis in a murine hindlimb ischemia model. (March 2017)

• Record Type: Journal Article
• Authors: Hyoun Kim, Myoung; Kim, Seul-Gi; Guhn Kim, Chang; Kim, Dae-Weung
• Is Part Of: Applied radiation and isotopes. Volume 121(2017:Mar.)
• Page Start: 22
• Page End: 27
• Publication Date: 2017-03
• Subjects: Serine-aspartic acid-valine -- Tc-99 m -- TAMRA -- Angiogenesis -- Multimodal imaging; Radiology -- Periodicals; Radiation -- Industrial applications -- Periodicals; Nuclear chemistry -- Periodicals; Internet resource; Periodical; 660.298
• DOI: 10.1016/j.apradiso.2016.12.026
• Languages: English
• ISSNs: 0969-8043

Abstract

Abstract: The serine-aspartic acid-valine (SDV) peptide binds specifically to integrin αvβ3. We developed a Tc-99 m and TAMRA labeled peptide, Tc-99 m SDV-ECG-K-TAMRA for multimodal imaging of angiogenesis. Tc-99 m SDV-ECG-K-TAMRA was prepared in high yield (>96%) and showed low cytotoxicity. Tc-99 m tetrofosmin images 1 week after operation, revealed significantly decreased perfusion of the ischemic hindlimb, and the perfusion recovered gradually for 4 weeks. In contrast, Tc-99 m SDV-ECG-K-TAMRA uptake was maximal 1 week after the operation (ischemic-to-non-ischemic uptake ratio =5.03±1.01) and decreased gradually. The ischemic-to-non-ischemic ratio of Tc-99 m SDV-ECG-K-TAMRA and Tc-99 m tetrofosmin was strongly negatively correlated (r =−0.94). A postmortem analysis revealed increased angiogenesis markers and uptake of Tc-99 m SDV-ECG-K-TAMRA by ischemic tissue. Our in vivo and in vitro studies revealed substantial uptake of Tc-99 m SDV-ECG-K-TAMRA by ischemic tissue. Tc-99 m SDV-ECG-K-TAMRA could be a good candidate dual-modality imaging agent to assess angiogenesis. Highlights: Dual-modality imaging agent, Tc-99 m SDV-ECG-K-TAMRA was prepared for angiogenesis imaging. Increased uptake of Tc-99 m SDV-ECG-K-TAMRA in ischemic tissue was demonstrated by in vivo and in vitro imagings. Tc-99 m SDV-ECG-K-TAMRA could be a good candidate dual-modality imaging agent to assess angiogenesis.