Characterization and signaling pathway analysis of interferon-kappa in bovine. (February 2017)
- Record Type:
- Journal Article
- Title:
- Characterization and signaling pathway analysis of interferon-kappa in bovine. (February 2017)
- Main Title:
- Characterization and signaling pathway analysis of interferon-kappa in bovine
- Authors:
- Guo, Yongli
An, Dong
Liu, Ying
Bao, Jun
Luo, Xiuxin
Cheng, Xintong
Wang, Yujiao
Gao, Mingchun
Wang, Junwei - Abstract:
- Abstract: A bovine interferon-kappa (BoIFN-κ) gene was amplified, which encodes a protein of 215 amino acids sharing 63% identity with human IFN-κ. BoIFN-κ was demonstrated to have antiviral and antiproliferative activities. Moreover, BoIFN-κ was shown to be highly sensitive to trypsin, however, it remained stable despite changes in pH and temperature. Result showed that BoIFN-κ can bind with bovine type I IFN receptors, and the antiviral activity can be blocked by antibodies against type I IFN receptors or BoIFN-κ. Additionally, BoIFN-κ can induce the transcription of Mx1, ISG15 and ISG56 gene, as well as the expression of Mx1 protein. The NF-κB, ISRE, and BoIFN-β promoter can all be activated by BoIFN-κ. This study revealed that BoIFN-κ exhibits the typical characteristics of type I IFNs and exerts antiviral activity via activation of the JAK-STAT signaling pathway. Overall, these findings will enrich the current knowledge about IFN-κ and facilitate further research on the role of type I IFN in antiviral defense responses in bovine. Highlights: BoIFN-κ has the typical characterization of type I interferon. The second exon of BoIFN-κ gene encodes no amino acid. BoIFN-κ has antiviral activity and antiproliferative activity. BoIFN-κ can bind with bovine type I IFN receptors. BoIFN-κ exerts antiviral activity by activating the NF-κB, ISRE, and BoIFN-β promoter.
- Is Part Of:
- Developmental and comparative immunology. Volume 67(2017)
- Journal:
- Developmental and comparative immunology
- Issue:
- Volume 67(2017)
- Issue Display:
- Volume 67, Issue 2017 (2017)
- Year:
- 2017
- Volume:
- 67
- Issue:
- 2017
- Issue Sort Value:
- 2017-0067-2017-0000
- Page Start:
- 213
- Page End:
- 220
- Publication Date:
- 2017-02
- Subjects:
- Bovine interferon-kappa -- Antiviral activity -- Type I interferon receptor -- Interferon-stimulated genes -- JAK-STAT signaling pathway
Immunology -- Periodicals
Developmental immunology -- Periodicals
616.079 - Journal URLs:
- http://www.sciencedirect.com/science/journal/0145305X ↗
http://www.elsevier.com/journals ↗ - DOI:
- 10.1016/j.dci.2016.09.018 ↗
- Languages:
- English
- ISSNs:
- 0145-305X
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3579.051000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 7650.xml