Molecular characterization and function of the Prohibitin2 gene in Litopenaeus vannamei responses to Vibrio alginolyticus. (February 2017)

• Record Type: Journal Article
• Authors: Gu, Mei-mei; Kong, Jing-rong; Di-Huang, ; Peng, Ting; Xie, Chen-ying; Yang, Kai-yuan; Liu, Yuan; Wang, Wei-Na
• Is Part Of: Developmental and comparative immunology. Volume 67(2017)
• Page Start: 177
• Page End: 188
• Publication Date: 2017-02
• Subjects: Litopenaeus vannamei -- PHB2 -- ROS -- p53 -- RNAi -- subcellular localization -- luciferase reporter assays; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2016.10.004
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Prohibitin2 (PHB2), a potential tumor suppressor protein, plays important roles in inhibition of cell cycle progression, transcriptional regulation, apoptosis and the mitochondrial respiratory chain. To explore its potential roles in crustaceans' immune responses we have identified and characterized Lv PHB2, a 891 bp gene encoding a 297 amino acids protein in the shrimp Litopenaeus vanname i. Expression analyses showed that Lv PHB2 is expressed in all examined tissues, and largely present in cytoplasm, correlating with its known anti-oxidation function in mitochondria. Luciferase reporter assays showed that over-expression of Lv PHB2 could activate the p53 pathway, indicating that it might participate in apoptosis regulation. Quantitative real-time PCR revealed that infection with Vibrio alginolyticus induces its up-regulation in hepatopancreas. Moreover, RNAi knock-down of Lv PHB2 in vivo raises mortality rates of L. vannamei infected by V. alginolyticus, and affects expression of STAT3, Caspase3 and p53 genes. We found significantly higher reactive oxygen species production, DNA damage and apoptosis rates in Lv PHB2-silenced shrimp challenged with V. alginolyticus than in controls injected with a Green Fluorescent Protein-silencing construct. Our results suggest that Lv PHB2 plays a vital role in shrimp responses to V. alginolyticus infection through its participation in regulation of oxidants and apoptosis. Highlights: A crustacean PHB2 gene ( Lv PHB2) was identified in Litopenaeus vannamei. Lv PHB2 is distributed in mitochondria. The transcription of Lv PHB2 after V. alginolyticus challenge indicated that it is involved in pathogen infection. LvPHB2 may play an important role in shrimp innate immunity. The over-expression of Lv PHB2 could activate p53 pathway, which revealed it might participate in apoptosis regulation.