Facile synthesis of 55Fe-labeled well-dispersible hematite nanoparticles for bioaccumulation studies in nanotoxicology. (June 2016)

• Record Type: Journal Article
• Authors: Huang, Bin; Xiao, Lin; Yang, Liu-Yan; Ji, Rong; Miao, Ai-Jun
• Is Part Of: Environmental pollution. Volume 213(2016)
• Page Start: 801
• Page End: 808
• Publication Date: 2016-06
• Subjects: Bioaccumulation -- 55Fe -- Hematite nanoparticles -- Poly(acrylic acid) -- Stability; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2016.03.063
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Although water-dispersible engineered nanoparticles (ENPs) have a wide range of applications, the ENPs used in many nanotoxicological studies tend to form micron-sized aggregates in the exposure media and thus cannot reflect the toxicity of real nanoparticles. Here we described the synthesis of bare hematite nanoparticles (HNPs-0) and two poly(acrylic acid) (PAA)-coated forms (HNPs-1 and HNPs-2). All three HNPs were well dispersed in deionized water, but HNPs-0 quickly aggregated in the three culture media tested. By contrast, the suspensions of HNPs-1 and HNPs-2 remained stable, with negligible amounts of PAA and Fe 3+ liberated from either one under the investigated conditions. To better quantify the accumulation of the coated HNPs, a relatively innocuous 55 Fe-labeled form of HNPs-2 was synthesized as an example and its accumulation in three phytoplankton species was tested. Consistent with the uptake kinetics model for conventional pollutants, the cellular accumulation of HNPs-2 increased linearly with exposure time for two of the three phytoplankton species. These results demonstrate the utility of 55 Fe-labeled well-dispersible HNPs as a model material for nanoparticle bioaccumulation studies in nanotoxicology. Graphical abstract: Highlights: The PAA-coated hematite nanoparticles were synthesized in two ways. They had good dispersibility and were innocuous to several organisms. Negligible amount of PAA and Fe 3+ was liberated from the nanoparticles. The method for 55 Fe-labeled hematite nanoparticle quantification was established. Their bioaccumulation followed the conventional biokinetics model. Abstract : 55 Fe-labeled well-dispersible hematite nanoparticles can serve as a model material for bioaccumulation studies in nanotoxicology.