A peculiar segmented flow microfluidics for isoquercitrin biosynthesis based on coupling of reaction and separation. (October 2015)

• Record Type: Journal Article
• Authors: Gong, An; Gu, Shuang-Shuang; Wang, Jun; Sheng, Sheng; Wu, Fu-An
• Is Part Of: Bioresource technology. Volume 193(2015)
• Page Start: 498
• Page End: 506
• Publication Date: 2015-10
• Subjects: Biphasic catalysis -- Enzymatic synthesis -- Isoquercitrin -- Micro-channel reactor -- Segmented flow; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.06.143
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A segmented flow microreactor containing "buffer-ionic liquid/solvent" was proposed. Isoquercitrin yield of 101.7 ± 2.6% using the microreactor was achieved in 20 min. The best reaction temperature of hesperidinase decreased from 40 to 30 °C. Reaction rate was improved to 4-fold than that in a continuous flow microreactor. Hesperidinase could be efficiently reused 5 times in a segmented flow microreactor. Abstract: A segmented flow containing a buffer-ionic liquid/solvent in a micro-channel reactor was applied to synthesize isoquercitrin by the hesperidinase-catalyzed selective hydrolysis of rutin, based on a novel system of reaction coupling with separation. Within the developed microchannel reactor with one T-shaped inlet and outlet, the maximum isoquercitrin yield (101.7 ± 2.6%) was achieved in 20 min at 30 °C and 4 μL/min. Compared with a continuous-flow reactor, reaction rate was increased 4-fold due to a glycine–sodium hydroxide:[Bmim][BF4 ]/glycerol triacetate (1:1, v/v) system that formed a slug flow in microchannel and significantly increased mass transfer rates. The mass transfer coefficient significantly increased and exhibited a linear relationship with the flow rate. Hesperidinase could be efficiently reused at least 5 times, without losing any activity. The bonding mechanism and secondary structure of hesperidinase indicated that hesperidinase had a greater affinity to rutin at a production rate of 4 μL/min in this segmented flow microreactor.