Effect of the molecular structure of lignin-based polyoxyethylene ether on enzymatic hydrolysis efficiency and kinetics of lignocelluloses. (October 2015)

• Record Type: Journal Article
• Authors: Lin, Xuliang; Qiu, Xueqing; Zhu, Duming; Li, Zihao; Zhan, Ningxin; Zheng, Jieyi; Lou, Hongming; Zhou, Mingsong; Yang, Dongjie
• Is Part Of: Bioresource technology. Volume 193(2015)
• Page Start: 266
• Page End: 273
• Publication Date: 2015-10
• Subjects: PEG polyethylene glycol -- EHL enzymatic hydrolysis lignin -- EHL–PEG copolymer of enzymatic hydrolysis lignin and PEG -- Mw weight-average molecular weight -- QCM-D quartz crystal microbalance with dissipation monitoring -- AFM atomic force microscopy -- Δf3 the frequency shift of the third overtone -- ΔD the energy dissipation shift of the third overtone -- Dmax the maximum energy dissipation -- SED substrate enzymatic digestibility; Lignin-based polyoxyethylene ether -- Avicel -- Corn stover -- Enzymatic hydrolysis -- Kinetic model; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.06.089
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: With increase of PEG contents and M w of EHL–PEG, glucose yield increased. EHL–PEG improved glucose yield of corn stover at 20% (w/v) from 32.8% to 63.8%. Effect of EHL–PEG on hydrolysis kinetics of cellulose was studied by QCM-D. Dispersed cellulase aggregates excavated cavities and peeled off cellulose film. Abstract: Effect of the molecular structure of lignin-based polyoxyethylene ether (EHL–PEG) on enzymatic hydrolysis of Avicel and corn stover was investigated. With the increase of PEG contents and molecular weight of EHL–PEG, glucose yield of corn stover increased. EHL–PEG enhanced enzymatic hydrolysis of corn stover significantly at buffer pH 4.8–5.5. Glucose yield of corn stover at 20% solid content increased from 32.8% to 63.8% by adding EHL–PEG, while that with PEG4600 was 54.2%. Effect of EHL–PEG on enzymatic hydrolysis kinetics of cellulose film was studied by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). An enhancing mechanism of EHL–PEG on enzymatic hydrolysis kinetics of cellulose was proposed. Cellulase aggregates dispersed by EHL–PEG excavated extensive cavities into the surface of cellulose film, making the film become more loose and exposed. After the maximum enzymatic hydrolysis rate, the film was mainly peeled off layer by layer until equilibrium.