Biosynthesis of (−)-ent-kaurenoic acid in Smallanthus sonchifolius and its effect against microbial biofilms. (December 2016)

• Record Type: Journal Article
• Authors: Lopes, Adriana A.; Pina, Edieidia S.; Nader, Talita T.; Da Costa, Fernando B.; Pereira, Ana Maria S.; Pupo, Mônica T.
• Is Part Of: Phytochemistry letters. Volume 18(2016:Dec.)
• Page Start: 162
• Page End: 167
• Publication Date: 2016-12
• Subjects: Diterpene -- Ent-kaurenoic acid -- Biosynthesis -- Methylerythritol-4-phosphate (MEP) pathway -- Smallanthus sonchifolius; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2016.10.011
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: Biosynthesis of (–)- ent -kaurenoic acid proceeds via MEP pathway in Smallanthus sonchifolis plantlets. Biosynthesis of different classes of terpenes are likely not be compartmentalized into cytosol and plastids. (–)- Ent -kaurenoic acid showed antimicrobial activity against Staphylococcus aureus biofilm. Abstract: The biosynthetic pathway of (–)- ent -kaurenoic acid (1 ) was investigated by incorporation of 1-d - 13 C-glucose in Smallanthus sonchifolius (Asteraceae) plantlets. The 13 C-enrichment pattern indicated that methylerythritol-4-phosphate (MEP) pathway is the biosynthetic pathway involved in diterpenoid biosynthesis. Our studies in S. sonchifolius reinforce that the biosynthesis of different classes of terpenes should not be compartmentalized into cytosol and plastids. Additionally, (–)- ent -kaurenoic acid showed antimicrobial activity against Staphylococcus aureus biofilm.