Dexamethasone Controlled Release on TGF-β1 Treated Vocal Fold Fibroblasts. (July 2015)

• Record Type: Journal Article
• Authors: Kosinski, Aaron M.; Pothen, Jewel M.; Panitch, Alyssa; Sivasankar, M. Preeti
• Is Part Of: Annals of otology, rhinology & laryngology. Volume 124:Number 7(2015:Jul.)
• Page Start: 572
• Page End: 578
• Publication Date: 2015-07
• Subjects: dexamethasone -- drug delivery -- PLGA microparticles -- vocal fold scar; Otolaryngology -- Periodicals; 617.51
• DOI: 10.1177/0003489415570933
• Languages: English
• ISSNs: 0003-4894

Abstract

Objective: Corticosteroids may be beneficial in treating vocal fold scarring. Current drug delivery methods do not permit controlled corticosteroid release. Here we investigate the effects of poly-lactic-co-glycolic acid (PLGA) microparticles loaded with the corticosteroid dexamethasone in reducing collagen synthesis and inflammation in vocal fold fibroblasts treated with and without TGF-β1. Study Design: Experimental, in vitro study. Methods: PLGA microparticles of differing molecular weight and terminating moieties were synthesized using a hydrogel template method. The release of dexamethasone was characterized from these microparticles over 4 days. Based on the release studies, ester-terminated low molecular weight PLGA microparticles were loaded with dexamethasone and applied to TGF-β1 treated vocal fold fibroblasts for 4 days. Quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assays (ELISAs) were used to assess the effects of released dexamethasone on collagen synthesis and inflammatory mediators. Results: COL3A1 and COL1A2 were significantly down-regulated after exposure to ester-terminated low molecular weight PLGA microparticles loaded with dexamethasone. The loaded microparticles also reduced interleukin-6 synthesis. Conclusion: These data show promise in using a PLGA microparticle-based delivery system to control dexamethasone release over 4 days. Our findings lay the groundwork for developing more effective treatments for vocal fold scarring.