Abscission-related genes revealed by RNA-Seq analysis using self-abscising apple (Malus × domestica). Issue 3 (3rd May 2016)

• Record Type: Journal Article
• Authors: Heo, S.; Hwang, J. H.; Jun, J. H.; Lee, H. J.
• Is Part Of: Journal of horticultural science & biotechnology. Volume 91:Issue 3(2016)
• Page Start: 271
• Page End: 278
• Publication Date: 2016-05-03
• Subjects: Malus x domestica -- apple -- RNA-seq -- self-abscission; Horticulture -- Periodicals; Plant biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology; Horticulture; Plant biotechnology; Periodicals; 635.05
• DOI: 10.1080/14620316.2016.1155315
• Languages: English
• ISSNs: 1462-0316

Abstract

ABSTRACT: The self-abscising characteristic of apple ( Malus × domestica ) fruit is a prominent labour-saving trait during cultivation. Early abscission up to 30 d after full bloom leaves only the central fruit in a cluster, while the four lateral fruit are abscised. A comparative analysis was conducted between the central and lateral pedicels in the self-abscising apple cultivar 'Saika' to identify the genes that trigger the abscission mechanism, as the destinies of the pedicels in a cluster are obvious. Transcriptome analysis was performed using RNA-Seq to compare expression profiles between the surviving central pedicel and the abscised lateral pedicels. A total of 797, 647 expressed sequence tags were assembled into 65, 876 contigs, which were annotated and analysed using Blast2GO. A total of 1, 585 differentially expressed genes were identified in the central and lateral pedicels using NOISeq software. Differentially expressed genes were confirmed by quantitative real-time reverse-transcription polymerase chain reaction analysis, focussing on the genes associated with auxin signalling. Our data suggested that the IAA14 / SLR gene is at least partly involved in the mechanism of abscission of the lateral pedicels.