GPER1 mediates estrogen-induced neuroprotection against oxygen-glucose deprivation in the primary hippocampal neurons. (22nd July 2016)

• Record Type: Journal Article
• Authors: Zhao, Tian-Zhi; Shi, Fei; Hu, Jun; He, Shi-Ming; Ding, Qian; Ma, Lian-Ting
• Is Part Of: Neuroscience. Volume 328(2016)
• Page Start: 117
• Page End: 126
• Publication Date: 2016-07-22
• Subjects: GPER1 (G Protein-coupled Estrogen Receptor 1) -- OGD oxygen-glucose deprivation -- PVN paraventricular nucleus; G Protein-coupled Estrogen Receptor 1 -- estrogen -- ischemia -- oxygen-glucose deprivation -- PI3 kinase -- non-genomic estrogen signaling; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2016.04.026
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: OGD stimulates GPER1 expression in primary hippocampal neurons. Membrane-initialized estrogen actions protect cell toxicity induced by OGD. GPER1 dependency of estrogen neuroprotective effects on hippocampal neurons. PI3K is required for the membrane-associated E2 function in neuroprotection. Abstract: It is well-known that the neuroprotective effects of estrogen have potential in the prevention and amelioration of ischemic and degenerative neurological disorders, while the underlying mechanisms for estrogen actions are undefined. As an important mediator for the non-genomic functions of estrogen, GPER1 (G Protein-coupled Estrogen Receptor 1) has been suggested to involve in the beneficial roles of estrogen in neural cells. Here our studies on primary hippocampal neurons have focused on GPER1 in an in vitro model of ischemia using oxygen-glucose deprivation (OGD). GPER1 expression in the primary hippocampal neurons was stimulated by the OGD treatments. Both E2 (estradiol) and E2-BSA (membrane impermeable estradiol by covalent conjugation of bovine serum albumin) attenuated OGD-induced cell death in primary cultures of hippocampal neurons. Importantly, this membrane-mediated estrogen function requires GPER1 protein. Knocking down of GPER1 diminished, while overexpression of GPER1 potentiated, the protective roles of E2/E2-BSA following OGD. Additionally, the downstream mechanisms employed by membrane-associated estrogen signaling were found to include PI3K/Akt-dependent Ask1 inhibition in the primary hippocampal neurons. Overall, these research results could enhance our understanding of the neuroprotective actions for estrogen, and provide a new therapeutic target for improving stroke outcome and ameliorating degenerative neurological diseases.