Bioaugmentation strategy employing a microbial consortium immobilized in chitosan beads for oil degradation in mesocosm scale. Issue 1 (15th June 2016)

• Record Type: Journal Article
• Authors: Dellagnezze, B.M.; Vasconcellos, S.P.; Angelim, A.L.; Melo, V.M.M.; Santisi, S.; Cappello, S.; Oliveira, V.M.
• Is Part Of: Marine pollution bulletin. Volume 107:Issue 1(2016:Jun.)
• Page Start: 107
• Page End: 117
• Publication Date: 2016-06-15
• Subjects: Bioaugmentation -- Mesocosm -- Metagenomic clones -- Consortium -- Petroleum; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2016.04.011
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: A bacterial consortium composed by four metagenomic clones and Bacillus subtilis strain CBMAI 707, all derived from petroleum reservoirs, was entrapped in chitosan beads and evaluated regarding hydrocarbon degradation capability. Experiments were carried out in mesocosm scale (3000 L) with seawater artificially polluted with crude oil. At different time intervals, mesocosms were sampled and subjected to GC-FID and microbiological analyses, as total and heterotrophic culturable bacterial abundance (DAPI and CFU count), biological oxygen demand (BOD) and taxonomic diversity (massive sequencing of 16S rRNA genes). The results obtained showed that degradation of n-alkane hydrocarbons was similar between both treatments. However, aromatic compound degradation was more efficient in bioaugmentation treatment, with biodegradation percentages reaching up to 99% in 30 days. Community dynamics was different between treatments and the consortium used in the bioaugmentation treatment contributed to a significant increase in aromatic hydrocarbon degradation. Highlights: A bacterial consortium used as bioaugmentation showed petroleum degradation potential. Degradation of some aromatic compounds was higher in bioaugmentation treatment after 30 days. Community dynamics was different between treatments, with a few groups favored in bioaugmentation.