Synthesis and structure formation in dilute aqueous solution of a chitosan-DNA hybrid. (19th November 2015)

• Record Type: Journal Article
• Authors: Safir, Ilyès; Ngo, Kien Xuan; Nixon Abraham, Jancy; Ghahraman Afshar, Majid; Pavlova, Ewa; Nardin, Corinne
• Is Part Of: Polymer. Volume 79(2015)
• Page Start: 29
• Page End: 36
• Publication Date: 2015-11-19
• Subjects: Chitosan -- DNA -- Self-assembly; Chitosan-grafted-single stranded DNA Chitosan-g-ssDNA -- degree of acetylation DA -- controlled pore glass CpG -- gel permeation chromatography GPC -- 2-(N-morpholino)ethanesulfonic acid buffer MES -- N-hydroxysuccinimide NHS -- 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride EDC -- matrix-assisted laser desorption/ionization time of flight MALDI-TOF -- 2, 5-dihydroxybenzoic acid matrix DHB -- analytical ultra-centrifugation AUC -- transmission electron microscopy TEM -- atomic force microscopy AFM -- poly(ethylene imine) PEI -- deacetylation degree DD -- ultraviolet UV -- right angle laser light scattering RALS -- low angle laser light scattering LALS -- refractive index RI -- tetrahydrofuran THF -- retention volume VR; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; 547.7
• DOI: 10.1016/j.polymer.2015.09.072
• Languages: English
• ISSNs: 0032-3861

Abstract

Abstract: In the following is described the combination of straight biochemical and organic routes to graft nucleotide sequences to a chitosan backbone. The resulting chitosan-g-ssDNA hybrid self-assembles into submicrometer size structures in dilute aqueous solution as assessed by atomic force and electron microscopy imaging. The hypothesis of self-assembly driven by chemical incompatibility between the amphiphilic chitosan and nucleic acid grafts is supported further by stability of the self-assembly against ionic strength and pH variations. Graphical abstract: Highlights: Low molecular weight covalent coupling of chitosan to DNA. Self-assembly into submicrometre size micelles. Stability against pH and ionic strength variations.