CD47 expression in cryopreserved equine cutaneous masses and normal skin. (July 2016)

• Record Type: Journal Article
• Authors: Caston, Stephanie S.; Cooper, Elizabeth E.; Chandramani-Shivalingappa, Prashanth; Sponseller, Brett A.; Hostetter, Jesse M.; Sun, Yaxuan
• Is Part Of: Journal of veterinary diagnostic investigation. Volume 28:Number 4(2016:Jul.)
• Page Start: 408
• Page End: 413
• Publication Date: 2016-07
• Subjects: CD47 -- equine -- immunohistochemistry -- macrophage -- neoplasia -- skin -- tumor; Veterinary medicine -- Diagnosis -- Periodicals; 636.0896075
• DOI: 10.1177/1040638716643352
• Languages: English
• ISSNs: 1040-6387

Abstract

We investigated CD47 expression in cryopreserved sections of equine cutaneous masses and normal skin. CD47 is a cell surface protein expressed on many cell types and overexpressed in some tumors. Interaction of CD47 and signal regulatory protein–alpha (SIRPα) inhibits phagocytosis by macrophages. Formalin-fixed tissues from horses prospectively enrolled in the study were used to establish a histologic diagnosis. Immunohistochemical assays were performed on cryopreserved tissues using anti-CD47 antibodies or IgG control antibodies. CD47 was not expressed on equine normal skin but positivity to CD47 was present in 13 of 24 (54%) masses. Immunotherapy with anti-CD47 antibodies for equine cutaneous tumors that express CD47 warrants further investigation.