Mapping of quantitative trait loci associated with rice black‐streaked dwarf virus disease and its insect vector in rice (Oryza sativa L.). (21st August 2018)

• Record Type: Journal Article
• Authors: Xu, Tingting; Liu, Yuqiang; Zhang, Le; Liu, Linglong; Wang, Chunming; Hu, Jinlong; Sun, Zhiguang; Pan, Gen; Xiao, Shizhuo; He, Jun; Huang, Jie; Qiu, Zeyu; Fan, Dejia; Jiang, Ling; Cheng, Xianian; Zhai, Huqu; Wan, Jianmin
• Editors: Ahn, Sang Nag
• Is Part Of: Plant breeding. Volume 137:Number 5(2018)
• Page Start: 698
• Page End: 705
• Publication Date: 2018-08-21
• Subjects: quantitative trait loci -- rice (Oryza sativa L.) -- rice black‐streaked dwarf virus disease -- small brown planthopper; Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12620
• Languages: English
• ISSNs: 0179-9541

Abstract

Abstract: Rice black‐streaked dwarf virus disease (RBSDVD), transmitted by small brown planthopper (SBPH, Laodelphax striatellus ), causes serious loss in rice production. Breeding resistant cultivars are one of the most effective strategies to control the virus disease and its vector. By both natural inoculations in the field and modified seedling‐box screening test in the glasshouse, an indica variety WR24 showed high resistance to RBSDVD and SBPH. An F2:3 population consisting of 153 lines derived from a cross between WR24 and a susceptible japonica variety Suyunuo was used for quantitative trait loci (QTL) analysis of RBSDVD and SBPH resistance. The linkage map consisting of 130 SSR markers was constructed with an average marker interval of 13.90 cM, spanning a total of 1890.9 cM. Totally, five QTLs for RBSDV resistance, viz. qRBSDV3 WR 24, qRBSDV6 WR 24, qRBSDV7 WR 24, qRBSDV9 WR 24 and qRBSDV11 WR 24, were detected on chromosomes 3, 6, 7, 9 and 11, with LOD scores of 2.7, 3.08, 3.13, 5.28 and 3.7, respectively. Meanwhile, three QTLs for SBPH resistance, including qSBPH5 WR 24, qSBPH7 WR 24 and qSBPH10 WR 24, were mapped on chromosomes 5, 7 and 10, with LOD scores of 2.18, 3.5 and 3.57, respectively. All resistant alleles were from WR24. Among these QTLs, qRBSDV7 WR 24, qSBPH5 WR 24 and qSBPH10 WR 24 were newly reported, and qSBPH10 WR 24 showed major effect that explained 17.9% of total phenotypic variance. The RBSDVD and SBPH resistance QTLs and the tightly linked DNA markers can be utilized in RBSDV and SBPH resistance breeding in rice.