The NADPH Oxidase Inhibitor Apocynin Suppresses Preneoplastic Liver Foci of Rats. (June 2017)

• Record Type: Journal Article
• Authors: Fuji, Satoshi; Suzuki, Shugo; Naiki-Ito, Aya; Kato, Hiroyuki; Hayakawa, Masashi; Yamashita, Yoriko; Kuno, Toshiya; Takahashi, Satoru
• Is Part Of: Toxicologic pathology. Volume 45:Number 4(2017)
• Page Start: 544
• Page End: 550
• Publication Date: 2017-06
• Subjects: nicotinamide adenine dinucleotide phosphate (NADPH) oxidase -- apocynin -- glutathione S-transferase placental form (GST-P)-positive foci -- reactive oxygen species (ROS) -- hepatocarcinogenesis -- rat; Pathology -- Periodicals; Toxicology -- Periodicals; Pathology; Toxicology; 615.9
• DOI: 10.1177/0192623317710013
• Languages: English
• ISSNs: 0192-6233

Abstract

Reactive oxygen species (ROS) have been revealed to be important factors for carcinogenesis and tumor progression. Therefore, we focused on an ROS-generating protein, nicotinamide adenine dinucleotide phosphate oxidase, and evaluated whether its inhibitor, apocynin, could suppress hepatocarcinogenesis in a medium-term rat liver bioassay. The number and size of glutathione S-transferase placental form (GST-P)-positive foci were significantly reduced by apocynin in a dose-dependent manner. The reduction of ROS generation by apocynin was confirmed by dihydroethidium staining. Apocynin treatment also significantly reduced Ki-67 positivity, downregulated cyclooxygenase 2, and suppressed the activation of the c-Myc pathway. Meanwhile, ROS generation was not different between GST-P-positive foci and surrounding GST-P-negative areas of the liver. In conclusion, the present data suggest that apocynin possesses a potential antihepatocarcinogenic property.