Characterization of inter-Sertoli cell tight and gap junctions in the testis of turtle: Protect the developing germ cells from an immune response. (October 2018)

• Record Type: Journal Article
• Authors: Ahmed, Nisar; Yang, Ping; Chen, Hong; Ujjan, Imtiaz Ali; Haseeb, Abdul; Wang, Lingling; Soomro, Feroza; Faraz, Shahid; Sahito, Benazir; Ali, Waseem; Chen, Qiusheng
• Is Part Of: Microbial pathogenesis. Volume 123(2018)
• Page Start: 60
• Page End: 67
• Publication Date: 2018-10
• Subjects: CLDN11 -- Cx43 -- Testis -- Spermatogenesis -- Immune response -- Chinese soft-shelled turtle; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.06.037
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: It is conceivable that early developing germ cells must across the basal to the luminal region of seminiferous tubules (STs) during spermatogenesis is associated with extensive restructuring of junctional complex. However, very limited information is documented about these junctional complexes in reptiles. In the present study we have determined the localization of inter-Sertoli cell tight junctions (TJ's), protein CLDN11 and gap junction protein Cx43 during spermatogenesis in the testis. In early spermatogenesis, weak immunoreactivity of CLDN11and focal localization of Cx43 was observed around the Sertoli cell in the luminal region, but completely delaminated from the basal compartment of STs. In late spermatogenesis, strong focal to linear localization of CLDN11and Cx43 was detected at the points of contact between two Sertoli cells and around the early stages of primary spermatocytes in the basal compartment of STs. In late spermatogenesis, localization of CLDN11and Cx43 was drastically reduced and seen only around Sertoli cells and spermatogonia near the basal lamina. However, transmission electron microscopy revealed that inter-Sertoli cell tight junctions were present within the basal compartment of STs, leaving the spermatogonia and early primary spermatocytes in the basal region during mid spermatogenesis. Gap junctions were observed between Sertoli cells, and Sertoli cells with spermatogonia and primary spermatocytes throughout spermatogenesis. Moreover, adherens and hemidesmosomes junctions were observed during spermatogenesis. The above findings collectively suggest that the intensity and localization of TJ's and gap junctions vary according to the spermatogenetic stages that might be protected the developing germ cells from own immune response. Highlights: This is the first study provide information about the tight and gap junction's location during spermatogenesis in turtle. Intensity of tight junctions varies during spermatogenesis according to different stages of developing germ cells. We determined that the gap junctions are highly associated with the development of inter-Sertoli cell tight junctions.