Circular RNA hsa_circ_0008344 regulates glioblastoma cell proliferation, migration, invasion, and apoptosis. Issue 7 (24th April 2018)

• Record Type: Journal Article
• Authors: Zhou, Jinxu; Wang, Hongxiang; Chu, Junsheng; Huang, Qilin; Li, Guangxu; Yan, Yong; Xu, Tao; Chen, Juxiang; Wang, Yuhai
• Is Part Of: Journal of clinical laboratory analysis. Volume 32:Issue 7(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-04-24
• Subjects: apoptosis -- glioblastoma -- hsa_circ_0008344 -- invasion -- proliferation; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.22454
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract : Background: Recent studies have found circular RNAs (circRNAs) involved in the biological process of cancers. However, little is known about their functional roles in glioblastoma. Methods: Human circRNA microarray analysis was performed to screen the expression profile of circRNAs in IDH1 wild‐type glioblastoma tissue. The expression of hsa_circ_0008344 in glioblastoma and normal brain samples was quantified by qRT‐PCR. Functional experiments were performed to investigate the biological functions of hsa_circ_0008344, including MTT assay, colony formation assay, transwell assay, and cell apoptosis assay. Results: CircRNA microarray revealed a total of 417 abnormally expressed circRNAs (>1.5‐fold, P < .05) in glioblastoma tissue compared with the adjacent normal brain. Hsa_circ_0008344, among the top differentially expressed circRNAs, was significantly upregulated in IDH1 wild‐type glioblastoma. Further in vitro studies showed that knockdown of hsa_circ_0008344 suppressed glioblastoma cell proliferation, colony formation, migration, and invasion, but increased cell apoptotic rate. Conclusions: Hsa_circ_0008344 is upregulated in glioblastoma and may contribute to the progression of this malignancy.