A novel exonuclease-assisted isothermal nucleic acid amplification with ultrahigh specificity mediated by full-length Bst DNA polymerase. Issue 75 (1st August 2018)

• Record Type: Journal Article
• Authors: Ye, Xin; Li, Yang; Wang, Lijuan; Fang, Xueen; Kong, Jilie
• Is Part Of: Chemical communications. Volume 54:Issue 75(2018)
• Page Start: 10562
• Page End: 10565
• Publication Date: 2018-08-01
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc04577a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A novel exonuclease-assisted isothermal amplification to amplify and determine nucleic acids very sensitively and with ultrahigh specificity. Abstract : Here, we introduced a novel exonuclease-assisted isothermal nucleic acid amplification (Exo-NAT) utilizing the full-length Bst DNA polymerase combined with a melting curve analysis. This method achieved an ultrahigh specificity with a good detection limit (10 2 copies) in both singleplex and multiplex detection, which was validated by detecting three diarrhea-inducing pathogens, rotavirus A, astrovirus, and adenovirus, in 42 clinical samples.