Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida. (September 2016)

• Record Type: Journal Article
• Authors: Karray, Raida; Hamza, Manel; Sayadi, Sami
• Is Part Of: Bioresource technology. Volume 216(2016)
• Page Start: 622
• Page End: 628
• Publication Date: 2016-09
• Subjects: Enzymatic pretreatment -- Green macroalgae Ulva rigida -- β-Glucosidase production -- Biogas production; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.05.067
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Green macroalgae Ulva rigida was used as feedstock for biogas production and as nitrogen source for A. niger submerged fermentation. The macroalgae is an efficient organic nitrogen source. The enzymatic cocktail which produced with Ulva rigida enhances the biogas production to 1175 mL g −1 CODint . Abstract: Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition. The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars. In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source. The cocktail is rich on β-glucosidase, pectinase and carboxy-methyl-cellulase (CMCase). The highest activity was obtained with β-glucosidase (109 IU mL −1 ) and pectinase (76 IU mL −1 ), while CMCase present the lowest activity 4.6 IU mL −1 . The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175 mL g CODint −1 .