Long-term production of bioethanol in repeated-batch fermentation of microalgal biomass using immobilized Saccharomyces cerevisiae. (November 2016)

• Record Type: Journal Article
• Authors: El-Dalatony, Marwa M.; Kurade, Mayur B.; Abou-Shanab, Reda A.I.; Kim, Hoo; Salama, El-Sayed; Jeon, Byong-Hun
• Is Part Of: Bioresource technology. Volume 219(2016)
• Page Start: 98
• Page End: 105
• Publication Date: 2016-11
• Subjects: Microalgae -- Saccharification -- Repeated batch fermentation -- Bioethanol -- Immobilized yeast -- Energy recovery; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.07.113
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Sonication combined with enzyme hydrolysis achieved a 445 mg/g release of TRS. S . cerevisiae showed TRS consumption efficiency of 91–98%. SSF exhibited higher ethanol production (10.5 g/L) compared with SHF (8.48 g/L). Energy recovery improved through immobilized repeated batch fermentations. Regenerated beads achieved a fermentation efficiency of 79.5% for four cycles. Abstract: Separate hydrolysis fermentation (SHF) and simultaneous saccharification fermentation (SSF) processes were studied for bioethanol production from microalgal biomass. SSF was selected as an efficient process to enhance the bioethanol yield through repeated-batches using immobilized yeast cells. Combined sonication and enzymatic hydrolysis of Chlamydomonas mexicana generated 10.5 and 8.48 g/L of ethanol in SSF and SHF, respectively. Yeast utilized maximum portion of total reducing sugar (TRS) reaching a consumption efficiency of 91–98%. A bioethanol yield of 0.5 g/g (88.2% of theoretical yield) and volumetric productivity of 0.22 g/L/h was obtained after 48 h of SSF. Immobilized yeast cells enabled repetitive production of ethanol for 7 cycles displaying a fermentation efficiency up to 79% for five consecutive cycles. The maximum ethanol production was 9.7 g/L in 2nd–4th cycles. A total energy recovery of 85.81% was achieved from microalgal biomass in the form of bioethanol. Repeated-batch SSF demonstrated the possibility of cost-effective bioethanol production.