Waste cooking oil: A new substrate for carotene production by Blakeslea trispora in submerged fermentation. (March 2016)

• Record Type: Journal Article
• Authors: Nanou, Konstantina; Roukas, Triantafyllos
• Is Part Of: Bioresource technology. Volume 203(2016)
• Page Start: 198
• Page End: 203
• Publication Date: 2016-03
• Subjects: Waste cooking oil -- Blakeslea trispora -- Carotenes -- Oxidative stress -- Culture morphology; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.12.053
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: The production of carotenes from waste cooking oil by B. trispora was studied. Waste cooking oil was found to be suitable for carotene production. The specific activities of superoxide dismutase and catalase were studied. The oxidative stress induced by hydroperoxides and BHT increased carotene production. Blakeslea trispora formed only pellets during fermentation. Abstract: The objective of this study was to evaluate a waste, waste cooking oil (WCO) as substrate for carotene production by Blakeslea trispora in shake flask culture. WCO was found to be a useful substrate for carotene production. B. trispora formed only pellets during fermentation. The oxidative stress in B. trispora induced by hydroperoxides and BHT as evidenced by increase of the specific activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly the production of carotenes. The highest concentration of carotenes (2021 ± 75 mg/l or 49.3 ± 0.2 mg/g dry biomass) was obtained in culture grown in WCO (50.0 g/l) supplemented with CSL (80.0 g/l) and BHT (4.0 g/l). In this case the carotenes produced consisted of β-carotene (74.2%), γ-carotene (23.2%), and lycopene (2.6%). The external addition in the above medium glucose, Span 80, yeast extract, casein acid hydrolysate, l -asparagine, thiamine. HCl, KH2 PO4, and MgSO4 ·7H2 O did not improve the production of carotenes.