Lung endothelial cell-targeted peptide-guided bFGF promotes the regeneration after radiation induced lung injury. (November 2018)

• Record Type: Journal Article
• Authors: Guan, Dongwei; Mi, Junwei; Chen, Xi; Wu, Yonghui; Yao, Yuanjiang; Wang, Linjie; Xiao, Zhifeng; Zhao, Yannan; Chen, Bing; Dai, Jianwu
• Is Part Of: Biomaterials. Volume 184(2018)
• Page Start: 10
• Page End: 19
• Publication Date: 2018-11
• Subjects: Basic fibroblast growth factor (bFGF) -- Lung endothelial cell -- Radiation induced lung injury (RILI) -- Lung-targeted therapy -- Vascular endothelial cell apoptosis; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2018.08.061
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Basic fibroblast growth factor (bFGF) can protect the lung against radiation-induced pulmonary vascular endothelial apoptosis and subsequent radiation-induced lung injury (RILI). However, guiding bFGF to pulmonary vascular endothelial cells is a key determinant for the success of bFGF therapy. To improve the lung-targeting ability of bFGF, a lung endothelial cell-targeting peptide was fused to bFGF (LET-bFGF). An in vitro biological activity assay indicated that fusion of LET did not affect the bioactivity of bFGF. In addition, the fused protein showed superior lung-targeting ability following intravenous injection. Upon injecting LET-bFGF intravenously after thorax radiation, LET-bFGF could better protect against pulmonary vascular endothelial cell apoptosis as early as 4 h post-radiation. Compared with native bFGF, enhanced therapeutic effects of LET-bFGF were also observed in terms of decreased vascular abnormalities, disorganized lung structure, inflammatory cell migration, and lung density at 2 months post-radiation. Therefore, lung endothelial cell-targeted bFGF may represent a promising remedy for RILI.