SWATH-MS in proteomics: current status. (2015)

• Record Type: Journal Article
• Authors: Wang, Jing; Sheng, Quanhu; Shyr, Yu
• Is Part Of: International journal of computational biology and drug design. Volume 8:Number 3(2015)
• Page Start: 192
• Page End: 202
• Publication Date: 2015
• Subjects: SWATH-MS -- proteomes -- sequential window acquisition -- fragment ion spectra -- tandem mass spectrometry -- computational biology -- drug design -- protein identification -- proteome identification -- protein quantification -- proteome quantification -- bioinformatics; Computational biology -- Periodicals; Drugs -- Design -- Periodicals; 570.285
• Languages: English
• ISSNs: 1756-0756

Abstract

Sequential window acquisition of all theoretical fragment ion spectra coupled to tandem mass spectrometry (SWATH-MS) is a new strategy for identification and quantification of proteins and proteomes proposed in 2012. SWATH-MS operates in unbiased data-independent acquisition (DIA) mode and combines the advantages of two widely used MS-based techniques: shotgun (high throughput) and SRM proteomics (high sensitivity and reproducibility). In this review, we provide an introduction of the SWATH-MS method, its application, and a perspective on the future of this powerful technology.