Oxygenation measurement by multi‐wavelength oxygen‐dependent phosphorescence and delayed fluorescence: catchment depth and application in intact heart. Issue 8 (22nd September 2014)

• Record Type: Journal Article
• Authors: Balestra, Gianmarco M.; Aalders, Maurice C.G.; Specht, Patricia A.C.; Ince, Can; Mik, Egbert G.
• Is Part Of: Journal of biophotonics. Volume 8:Issue 8(2015)
• Page Start: 615
• Page End: 628
• Publication Date: 2014-09-22
• Subjects: oxygen‐dependent quenching -- phosphorescence -- delayed fluorescence -- oxygen measurement -- myocardial -- protoporphyrin IX -- Oxyphor G2 -- Monte Carlo simulation -- catchment depth; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201400054
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : Oxygen delivery and metabolism represent key factors for organ function in health and disease. We describe the optical key characteristics of a technique to comprehensively measure oxygen tension (PO2 ) in myocardium, using oxygen‐dependent quenching of phosphorescence and delayed fluorescence of porphyrins, by means of Monte Carlo simulations and ex vivo experiments. Oxyphor G2 (microvascular PO2 ) was excited at 442 nm and 632 nm and protoporphyrin IX (mitochondrial PO2 ) at 510 nm. This resulted in catchment depths of 161 (86) µm, 350 (307) µm and 262 (255) µm respectively, as estimated by Monte Carlo simulations and ex vivo experiments (brackets). The feasibility to detect changes in oxygenation within separate anatomical compartments is demonstrated in rat heart in vivo .Schematic of ex vivo measurements. Abstract : Oxygen delivery and metabolism represent key factors for organ function in health and disease. The optical key characteristics of a technique to comprehensively measure oxygen tension (PO2 ) in myocardium is described. Oxygen‐dependent quenching of phosphorescence and delayed fluorescence of porphyrins by means of Monte Carlo simulations and ex vivo experiments are used. The feasibility to detect changes in oxygenation within separate anatomical compartments is demonstrated in rat heart in vivo .