Sequencing Mirror-Image DNA Chemically. Issue 9 (20th September 2018)

• Record Type: Journal Article
• Authors: Liu, Xianyu; Zhu, Ting F.
• Is Part Of: Cell chemical biology. Volume 25:Issue 9(2018)
• Page Start: 1151
• Page End: 1156.e3
• Publication Date: 2018-09-20
• Subjects: L-DNA -- Maxam-Gilbert sequencing -- L-aptamer -- chirality; Biochemistry -- Periodicals; 572.05
• DOI: 10.1016/j.chembiol.2018.06.005
• Languages: English
• ISSNs: 2451-9456

Abstract

Summary: The development of mirror-image biology systems faces a crucial barrier of lacking an L-DNA sequencing technique. Here, we developed a practical method for sequencing mirror-image DNA by adopting the Maxam-Gilbert sequencing approach, through which specific nucleobases in an end-labeled L-DNA are cleaved by achiral chemicals. This technique may facilitate the therapeutic application of nuclease-resistant L-aptamer drugs, and bring the vision of building an alternative, mirror-image self-replicating system closer to reality. Graphical Abstract: Highlights: Developing a practical method for L-DNA sequencing Applying the classic Maxam-Gilbert sequencing method to mirror-image biology systems Sequencing nuclease-resistant L-DNA aptamers for therapeutic applications Abstract : A key technical hurdle in the development of mirror-image biology systems is the lack of a practical L-DNA sequencing approach. Liu and Zhu developed a method for sequencing mirror-image DNA by adopting the classic Maxam-Gilbert approach through cleavage of end-labeled L-DNA by achiral chemicals.