The mechanism of DNA damage induced by Roundup 360 PLUS, glyphosate and AMPA in human peripheral blood mononuclear cells - genotoxic risk assessement. (October 2018)

• Record Type: Journal Article
• Authors: Woźniak, Ewelina; Sicińska, Paulina; Michałowicz, Jaromir; Woźniak, Katarzyna; Reszka, Edyta; Huras, Bogumiła; Zakrzewski, Jerzy; Bukowska, Bożena
• Is Part Of: Food and chemical toxicology. Volume 120(2018)
• Page Start: 510
• Page End: 522
• Publication Date: 2018-10
• Subjects: Glyphosate -- Roundup 360 PLUS -- PBMCs -- DNA bases -- DNA adducts -- Reactive oxygen species; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.07.035
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Glyphosate is the most heavily applied among pesticides in the world, and thus human exposure to this substance continues to increase. WHO changed classification of glyphosate to probably cancerogenic to humans, thus there is urgent need to assess in detail genotoxic mechanism of its action. We have assessed the effect of glyphosate, its formulation (Roundup 360 PLUS) and its main metabolite (aminomethylphosphonic acid, AMPA) in the concentration range from 1 to 1000 μM on DNA damage in human peripheral blood mononuclear cells (PBMCs). The cells were incubated for 24 h. The compounds studied and formulation induced DNA single and double strand-breaks and caused purines and pyrimidines oxidation. None of compounds examined was capable of creating adducts with DNA, while those substances increased ROS (including OH) level in PBMCs. Roundup 360 PLUS caused damage to DNA even at 5 μM, while glyphosate and particularly AMPA induced DNA lesions from the concentration of 250 μM and 500 μM, respectively. DNA damage induced by glyphosate and its derivatives increased in order: AMPA, glyphosate, Roundup 360 PLUS. We may conclude that observed changes were not associated with direct interaction of xenobiotics studied with DNA, but the most probably they occurred through ROS–mediated effects. Highlights: Glyphosate, Roundup 360 PLUS and AMPA induced DNA single and double strand-breaks. The compounds studied caused purines and pyrimidines oxidation. Glyphosate, Roundup and AMPA increased ROS level in PBMCs. Glyphosate and Roundup increased . OH level in peripheral blood mononuclear cells. The compounds examined were not capable of creating adducts with DNA.