Antimicrobial photodynamic active biomaterials for periodontal regeneration. Issue 10 (October 2018)

• Record Type: Journal Article
• Authors: Sigusch, B.W.; Dietsch, S.; Berg, A.; Voelpel, A.; Guellmar, A.; Rabe, U.; Schnabelrauch, M.; Steen, D.; Gitter, B.; Albrecht, V.; Watts, D.C.; Kranz, S.
• Is Part Of: Dental materials. Volume 34:Issue 10(2018)
• Page Start: 1542
• Page End: 1554
• Publication Date: 2018-10
• Subjects: Biomaterial -- Laser -- Photodynamic therapy -- Periodontitis -- Periodontal bone regeneration -- aPDT -- Photosensitizer -- Enterococcus faecalis -- Porphyromonas gingivalis; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2018.06.026
• Languages: English
• ISSNs: 0109-5641

Abstract

Abstract: Objective: Biomaterials for periodontal regeneration may have insufficient mechanical and antimicrobial properties or are difficult to apply under clinical conditions. The aim of the present study was to develop a polymeric bone grafting material of suitable physical appearance and antimicrobial photodynamic activity. Methods: Two light curable biomaterials based on urethane dimethacrylate (BioM1) and a tri-armed oligoester-urethane methacrylate (BioM2) that additionally contained a mixture of β-tricalcium phosphate microparticles and 20 wt% photosensitizer mTHPC (PS) were fabricated and analyzed by their compressive strength, flexural strength and modulus of elasticity. Cytotoxicity was observed by incubating eluates and in direct-contact to MC3T3-E1 cells. Antimicrobial activity was ascertained on Porphyromonas gingivalis and Enterococcus faecalis upon illumination with laser light (652 nm, 1 × 100 J/cm 2, 2 × 100 J/cm 2 ). Results: The compressive strength, flexural strength and elastic modulus were, respectively, 311.73 MPa, 22.81 MPa and 318.85 MPa for BioM1 + PS and 742.37 MPa, 7.58 MPa and 406.23 MPa for BioM2 + PS. Both materials did not show any cytotoxic behavior. Single laser-illumination (652 nm) caused total suppression of P. gingivalis (BioM2 + PS), while repeated irradiation reduced E. faecalis by 3.7 (BioM1 + PS) and 3.1 (BioM2 + PS) log-counts. Significance: Both materials show excellent mechanical and cytocompatible properties. In addition, irradiation with 652 nm induced significant bacterial suppression. The manufactured biomaterials might enable a more efficient cure of periodontal bone lesions. Due to the mechanical properties functional stability might be increased. Further, the materials are antimicrobial upon illumination with light that enables a trans-mucosal eradication of residual pathogens.