Transcriptomic, lipid, and histological profiles suggest changes in health in fish from a pesticide hot spot. (September 2018)

• Record Type: Journal Article
• Authors: Hook, Sharon E.; Mondon, Julie; Revill, Andrew T.; Greenfield, Paul A.; Smith, Rachael A.; Turner, Ryan D.R.; Corbett, Patricia A.; Warne, Michael St J.
• Is Part Of: Marine environmental research. Volume 140(2018)
• Page Start: 299
• Page End: 321
• Publication Date: 2018-09
• Subjects: Atrazine -- Diuron -- Imidacloprid -- RNA Seq -- Asian sea bass (barramundi) -- Agricultural pollution -- Lipids -- Pesticides; Marine pollution -- Environmental aspects -- Periodicals; Marine ecology -- Periodicals; Mer -- Pollution -- Aspect de l'environnement -- Périodiques; Écologie marine -- Périodiques; Electronic journals; 577.705
• DOI: 10.1016/j.marenvres.2018.06.020
• Languages: English
• ISSNs: 0141-1136

Abstract

Abstract: Barramundi ( Lates calcarifer ) were collected at the beginning (1st sampling) and end (2nd sampling) of the wet season from Sandy Creek, an agriculturally impacted catchment in the Mackay Whitsundays region of the Great Barrier Reef catchment area, and from Repulse Creek, located approximately 100 km north in Conway National Park, to assess the impacts of pesticide exposure. Gill and liver histology, lipid class composition in muscle, and the hepatic transcriptome were examined. The first sample of Repulse Creek fish showed little tissue damage and low transcript levels of xenobiotic metabolism enzymes. Sandy Creek fish showed altered transcriptomic patterns, including those that regulate lipid metabolism, xenobiotic metabolism, and immune response; gross histological alterations including lipidosis; and differences in some lipid classes. The second sampling of Repulse Creek fish showed similar alterations in hepatic transcriptome and tissue structure as fish from Sandy Creek. These changes may indicate a decrease in health of pesticide exposed fish. Highlights: Fish from a pesticide hot spot were compared to those in fish from a national park. Fish from the hotspot had alterations in histology, transcriptome and some lipid classes. Fish from the second collection from the national park showed similar alterations. The physiological alterations may coincide with pesticide exposure or health declines.