200 mm wafer-scale fabrication of polydimethylsiloxane fluidic devices for fluorescence imaging of single DNA molecules. (11th April 2018)
- Record Type:
- Journal Article
- Title:
- 200 mm wafer-scale fabrication of polydimethylsiloxane fluidic devices for fluorescence imaging of single DNA molecules. (11th April 2018)
- Main Title:
- 200 mm wafer-scale fabrication of polydimethylsiloxane fluidic devices for fluorescence imaging of single DNA molecules
- Authors:
- Nam, Sung-Wook
- Abstract:
- Abstract: Abstract : We report fabrication of 200 mm silicon (Si)-wafer mold structure for polydimethylsiloxane (PDMS) microfluidic devices to demonstrate a real-time fluorescence imaging of single DNA molecules. Conventional photolithography with deep reactive ion etching process allows us to build a "mesa"-type Si mold with a nanoscallop sidewall geometry aiding PDMS residue-free process. By optimizing fluorescence microscopy with the fabricated PDMS chamber, we obtain a protocol to visualize the motions of single DNA molecules. This integrative PDMS-based single-molecule imaging system can, in principle, be used as a platform to study biochemical reactions occurring in proteins, nucleotides, and vesicles.
- Is Part Of:
- MRS communications. Volume 8:Number 2(2018)
- Journal:
- MRS communications
- Issue:
- Volume 8:Number 2(2018)
- Issue Display:
- Volume 8, Issue 2 (2018)
- Year:
- 2018
- Volume:
- 8
- Issue:
- 2
- Issue Sort Value:
- 2018-0008-0002-0000
- Page Start:
- 420
- Page End:
- 427
- Publication Date:
- 2018-04-11
- Subjects:
- Materials -- Periodicals
620.11 - Journal URLs:
- http://journals.cambridge.org/action/displayJournal?jid=MRC ↗
http://link.springer.com/ ↗ - DOI:
- 10.1557/mrc.2018.58 ↗
- Languages:
- English
- ISSNs:
- 2159-6859
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 7537.xml