Imaging‐Guided pHe and Glutathione Dual Responsive Polypeptide Nanogel for Smart Drug Delivery. Issue 9 (21st June 2018)

• Record Type: Journal Article
• Authors: Jing, Titao; Li, Tuanwei; Ruan, Zheng; Cheng, Quan; Yan, Lifeng
• Is Part Of: Macromolecular materials and engineering. Volume 303:Issue 9(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-06-21
• Subjects: glutathione -- imaging‐guided -- nanogels -- polypeptides -- tumor microenvironments; Plastics -- Periodicals; Polymers -- Periodicals; Polymerization -- Periodicals; 547.705
• DOI: 10.1002/mame.201800060
• Languages: English
• ISSNs: 1438-7492

Abstract

Abstract: A negatively charged polypeptide nanogel, near‐infrared (NIR) cyanine dye (Cy5.5) conjugated and 2, 3‐dimethylmaleic anhydride (DMA) modified poly‐l ‐lysine‐co‐l ‐cystine (CDPLC), is synthesized and is used as an imaging‐guided sequential drug delivery system. The CDPLC nanogel can respond to two general stimulations in sequence: extracellular tumor acidic microenvironment pHe (6.8–6.5) and intracellular high concentration glutathione (GSH). Under pHe, the DMA shell of the nanogel is removed and a charge reversal takes place, resulting in positively charged nanogel which can be internalized by cancer cells easily. Once internalized into tumor cells, the increased intracellular GSH concentration further promotes DOX release from the nanogel and DOX is enriched to the nucleus. Cy5.5 is conjugated to the nanogel as an NIR fluorescent probe, making it possible for imaging‐guided drug delivery, which is confirmed by the MTT and confocal laser scanning microscopy via in vitro experiments. The as‐prepared nanogel is a potential theranostic for cancer therapy. Abstract : Polypeptide‐based pHe and GSH sequential responsive nanogel conjugated Cy5.5 dye is synthesized which has potential application on NIR imaging‐guided chemotherapy.