Electrophysiological dysfunction induced by anti-ribosomal P protein antibodies injection into the lateral ventricle of the rat brain. (April 2017)

• Record Type: Journal Article
• Authors: Gaburo, N; de Carvalho, J Freire; Timo-Iaria, C; Bueno, C; Reichlin, M; Viana, V S T; Bonfá, E
• Other Names: Levy Roger A guest-editor.; de Carvalho Jozélio F guest-editor.
• Is Part Of: Lupus. Volume 26:Number 5(2017)
• Page Start: 463
• Page End: 469
• Publication Date: 2017-04
• Subjects: Systemic lupus erythematosus -- psychosis -- anti-P ribosomal antibody -- experimental model -- autoimmunity -- central nervous system; Systemic lupus erythematosus -- Periodicals; 616.772005
• DOI: 10.1177/0961203316666185
• Languages: English
• ISSNs: 0961-2033

Abstract

Objective: Anti-ribosomal P antibodies (anti-P) are strongly associated with neuropsychiatric lupus. This study was designed to determine whether these antibodies are capable of causing electro-oscillogram (EOSG) and behavior alterations in rats. Methods: IgG fraction anti-P positive and affinity-purified anti-P antibodies were injected intraventricularly in rats. Sequential cortical and subcortical EOSGs were analyzed during 30 days. IgG anti-Ro/SS-A and normal IgG were used as controls. Results: All 13 animals injected with IgG anti-P demonstrated a high prevalence of polyspikes, diffusely distributed in hippocampal fields and cerebral cortex. These abnormalities persisted approximately a month. Remarkably, an identical electrical disturbance was observed with the inoculation of affinity-purified anti-P antibodies. The EOSG alterations were associated with behavioral disorders with varying degrees of severity in every animal injected with anti-P. In contrast, no changes in EOSG or behavioral disturbances were observed in the control group. Conclusion: Our study indicates that anti-P antibodies can directly induce electrophysiological dysfunction in central nervous system particularly in hippocampus and cortex associated with behavior disturbances.