Indoleamine 2, 3-dioxygenase: First evidence of expression in rainbow trout (Oncorhynchus mykiss). (December 2016)

• Record Type: Journal Article
• Authors: Cortés, Jimena; Alvarez, Claudio; Santana, Paula; Torres, Elisa; Mercado, Luis
• Is Part Of: Developmental and comparative immunology. Volume 65(2016)
• Page Start: 73
• Page End: 78
• Publication Date: 2016-12
• Subjects: Tryptophan metabolism -- Fish IFN-γ -- Immune regulation -- ITIMs motifs -- Fish innate immunity; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2016.06.020
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: The role of enzymes as active antimicrobial agents of the innate immunity in teleost fish is proposed in diverse works. Secretion of Indoleamine 2, 3-dioxygenase (IDO) has been described in higher vertebrates; it degradesl -tryptophan in extracellular environments associated mainly with mucosal organs. The effect of IDO on decreasing amino acid concentration may inhibit the growth of potential pathogens. In fish the study of this molecule is still. Here we report the identification of an Onchorhyncus mykiss IDO homologue ( Om IDO). IDO was cloned, sequenced, and the primary structure shows conservation of key functional sites. The constitutive expression is altered when the fish is challenged with LPS as a pathogen-associated molecular pattern (PAMPs). Up-regulation of IDO was shown preferentially in the fish's mucosal cells. In order to obtain evidence of a possible regulation mechanism, an in vitro cell model was used for to show that Om IDO is induced by rIFN. These study has identified a Indoleamine 2, 3-dyoxigenase in O . mykiss will contribute to expands our knowledge of the function this protein in fish immune response. These findings allow to propose the use of OmIDO as a molecular indicator of strength of the animal's immune response and wellbeing. Highlights: The presence of Indoleamine 2, 3-dioxigenase in Oncorhynchus mykiss ( Om IDO) was demonstrated. Om IDO is expressed in different organs of the trout and up-regulated by LPS. rIFN-γ up-regulated Om IDO RNA in RTS-11 cell line.