A novel human-like collagen hemostatic sponge with uniform morphology, good biodegradability and biocompatibility. (March 2017)

• Record Type: Journal Article
• Authors: Jiang, Xijuan; Wang, Ya; Fan, Daidi; Zhu, Chenhui; Liu, Lin; Duan, Zhiguang
• Is Part Of: Journal of biomaterials applications. Volume 31:Number 8(2017:Mar.)
• Page Start: 1099
• Page End: 1107
• Publication Date: 2017-03
• Subjects: Human-like collagen -- glutamine transaminase -- freeze-drying -- uniform -- biodegradable -- biocompatibility -- hemostatic sponge; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1177/0885328216687663
• Languages: English
• ISSNs: 0885-3282

Abstract

Biodegradable sponges, as a promising hemostatic biomaterial, has been clinically required over the past decades. Current hemostatic sponges are generally prepared by crosslinking and freeze-drying, but the quality control or biocompatibility is often unsatisfactory due to the freezing-caused morphological non-uniformity and the toxicity of raw materials or cross-linkers. The crosslinking often greatly retards the degradation of the sponges and thus affects the healing of the wound. In this work, we prepared a novel hemostatic sponge using human-like collagen and glutamine transaminase (non-toxic cross-linker) and optimized its morphology via "two-step" freezing instead of conventional "one-step" freezing. The resulting sponge showed a good biocompatibility in cytotoxicity and implantation tests and had a significant hemostatic effect in ear artery and liver injury models. Moreover, the sponge could be degraded high efficiently by several common enzymes in organisms (e.g. I collagenase, trypsase, and lysozyme), which means that the sponge can be easily digested by metabolism and can facilitate seamless healing. Finally, both the front and back of the sponge prepared via two-step freezing was more uniform in morphology than that prepared via one-step freezing. More importantly, two-step freezing can be used as a universal approach for preparation of diverse uniform biomaterials.