Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes. Issue 27 (7th July 2016)
- Record Type:
- Journal Article
- Title:
- Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes. Issue 27 (7th July 2016)
- Main Title:
- Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes
- Authors:
- Li, Yi-Tong
Huang, Yi-Chao
Xu, Yang
Pan, Man
Li, Yi-Ming - Abstract:
- Abstract: A new fluorogenic substrate Ub-ACC (ubiquitin C-terminal 7-amino-4-carbamoylmethyl-coumarin) was developed for DUB (deubiquitinating enzyme) activity assays. This substrate can be synthesized with higher efficiency than the classical DUB substrate, Ub-AMC (ubiquitin C-terminal 7-amino-4-methylcoumarin). DUB assays using UCH-L3, OTUD2 and USP30 demonstrated that Ub-ACC shows nearly 2-fold higher sensitivity than Ub-AMC. Graphical abstract:
- Is Part Of:
- Tetrahedron. Volume 72:Issue 27/28(2016)
- Journal:
- Tetrahedron
- Issue:
- Volume 72:Issue 27/28(2016)
- Issue Display:
- Volume 72, Issue 27/28 (2016)
- Year:
- 2016
- Volume:
- 72
- Issue:
- 27/28
- Issue Sort Value:
- 2016-0072-NaN-0000
- Page Start:
- 4085
- Page End:
- 4090
- Publication Date:
- 2016-07-07
- Subjects:
- Ub-ACC -- Deubiquitinating enzyme -- Ub-AMC -- Peptide hydrazides -- Protease assay
Chemistry, Organic -- Periodicals
547.005 - Journal URLs:
- http://www.elsevier.com/journals ↗
- DOI:
- 10.1016/j.tet.2016.05.041 ↗
- Languages:
- English
- ISSNs:
- 0040-4020
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 8796.850000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 7515.xml