In vitro effects of deoxynivalenol and zearalenone major metabolites alone and combined, on cell proliferation, steroid production and gene expression in bovine small-follicle granulosa cells. (January 2016)

• Record Type: Journal Article
• Authors: Pizzo, Fabiola; Caloni, Francesca; Schreiber, Nicole B.; Cortinovis, Cristina; Spicer, Leon J.
• Is Part Of: Toxicon. Volume 109(2016)
• Page Start: 70
• Page End: 83
• Publication Date: 2016-01
• Subjects: Fusarium mycotoxins -- Progesterone -- Estradiol -- CYP11A1 -- CYP19A1 -- Cattle; α-zearalenol (α-Zol) -- β-zearalenol (β-Zol) -- de-epoxy deoxynivalenol (DOM1) -- deoxynivalenol (DON) -- estradiol (E2) -- estrogen receptor (ER) -- follicle-stimulating hormone (FSH) -- granulosa cells (GC) -- insulin-like growth factor (IGF) -- progesterone (P4) -- reverse transcriptase polymerase chain reaction (RT-PCR) -- radioimmunoassay (RIA) -- small-follicle granulosa cells (SMGC) -- zearalenone (ZEA); Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2015.11.018
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: The effects of deoxynivalenol (DON) and zearalenone (ZEA) on reproduction in ruminants are unclear. This study was performed to evaluate the impact of DON and ZEA hydroxylated metabolites, α-zearalenol (α-Zol) and β-zearalenone (β-Zol), on cell proliferation, steroidogenesis and gene expression using bovine granulosa cells (GC). Cell proliferation was negatively affected after exposure to β-Zol at 31 μM and after exposure to α-Zol (3.1 μM) alone and combined with DON (3.3 μM). DON and α-Zol decreased steroidogenesis, while β-Zol at high concentration had stimulatory effects. DON and β-Zol increased CYP19A1 mRNA abundance. CYP11A1 mRNA abundance was stimulated by DON, alone and combined with α-Zol and β-Zol, whereas was inhibited by β-Zol alone. Generally mycotoxins effects on cell proliferation, steroidogenesis and gene expression were influenced by the presence or absence of IGF1. In conclusion DON and ZEA metabolites may impair in vitro cell proliferation, steroid production and gene expression in cattle. Highlights: Granulosa cells were used to study the reproductive effects of Fusarium mycotoxins. Deoxynivalenol and zearalenone major metabolites were tested alone and in combination. Deoxynivalenol strongly inhibited estradiol and progesterone production. β-zearalenol impaired granulosa cells function stimulating hormones production. All the mycotoxins tested had the ability to affect in vitro granulosa cells function.