MicroRNA‐543 functions as an osteogenesis promoter in human periodontal ligament‐derived stem cells by inhibiting transducer of ERBB2, 2. (31st May 2018)

• Record Type: Journal Article
• Authors: Ge, Y.; Li, J.; Hao, Y.; Hu, Y.; Chen, D.; Wu, B.; Fang, F.
• Is Part Of: Journal of periodontal research. Volume 53:Number 5(2018)
• Page Start: 832
• Page End: 841
• Publication Date: 2018-05-31
• Subjects: microRNA‐543 -- osteogenesis -- periodontal ligament -- regeneration -- stem cell -- transducer of ERBB2, 2; Periodontics -- Periodicals; 617.632
• DOI: 10.1111/jre.12572
• Languages: English
• ISSNs: 0022-3484

Abstract

Abstract : Background and Objective: Previous research has indicated that altered expression of microRNAs (miRNAs) is in connection with osteogenesis of human periodontal ligament‐derived stem cells (hPDLSCs). We investigated the mechanisms by which miR‐543 promotes osteogenic differentiation of hPDLSCs. Material and Methods: First, the expression of miR‐543 in hPDLSCs cultured with or without an osteogenic inductive cocktail was explored. Then, the function of miR‐543 during osteogenesis of hPDLSCs was investigated by overexpressing and inhibiting miR‐543. Next, 3 databases were used to predict target genes of miR‐543 and a luciferase report was used to validate the direct regulation of miR‐543 on the target gene. Further, a rescue experiment using co‐transfection of miR‐543 mimic and target mimic was performed to evaluate whether overexpressing the target gene could partly rescue the efficiency of overexpressing miR‐543 on osteogenesis in hPDLSCs. Results: miR‐543 was upregulated during osteogenic differentiation of hPDLSCs. Functional experiments showed that overexpressing miR‐543 could enhance osteogenesis, while inhibiting miR‐543 resulted in reduced formation of mineralized nodules. The transducer of ERBB2, 2 (TOB2) was identified as a target gene of miR‐543 and luciferase report revealed that miR‐543 interacts directly with the 3′‐untranslated repeat sequence of TOB2 mRNA. Overexpression of miR‐543 inhibited the expression of TOB2 in both mRNA and protein levels while inhibiting miR‐543 increased. Furthermore, the rescue experiment confirmed the promotional role of miR‐543 TOB2 expression could be abrogated by overexpressing TOB2, which also had the effect of reducing osteogenic differentiation. Conclusion: Our research confirmed that miR‐543 is a promoter of osteogenesis in hPDLSCs, acting by inhibiting its target gene TOB2, which suggests that miR‐543 may be a potential therapy for bone loss in periodontitis.