Gingival crevicular fluid levels of human beta‐defensin 1 in individuals with and without chronic periodontitis. (23rd April 2018)

• Record Type: Journal Article
• Authors: Costa, L. C. M.; Soldati, K. R.; Fonseca, D. C.; Costa, J. E.; Abreu, M. H. N. G.; Costa, F. O.; Zandim‐Barcelos, D. L.; Cota, L. O. M.
• Is Part Of: Journal of periodontal research. Volume 53:Number 5(2018)
• Page Start: 736
• Page End: 742
• Publication Date: 2018-04-23
• Subjects: beta‐defensins -- chronic periodontitis -- ELISA (enzyme‐linked immunosorbent assay) -- gingival crevicular fluid; Periodontics -- Periodicals; 617.632
• DOI: 10.1111/jre.12558
• Languages: English
• ISSNs: 0022-3484

Abstract

Abstract : Background and Objective: Human beta‐defensins (hBDs) contribute to innate immunity antimicrobial activity. They are also effective in the adaptive immune response and may play a crucial role in the susceptibility to diseases of the oral cavity. This study aimed to evaluate the levels of hBD‐1 in the gingival crevicular fluid of individuals with and without chronic periodontitis. Material and Methods: Twenty periodontally healthy individuals (H) and 20 individuals with chronic periodontitis were recruited. Gingival crevicular fluid samples were collected from: healthy sites (Hh) from periodontally healthy individuals; and healthy sites (Ph), sites with gingivitis (Pg), and sites with periodontitis (Pp) from individuals with periodontitis. The levels of hBD‐1 (pg/mL) were measured using enzyme‐linked immunosorbent assay. Comparisons of hBD‐1 between individuals (H and chronic periodontitis) and among sites (Hh, Ph, Pg, Pp) were performed through hierarchical linear modeling. Results: Gingival crevicular fluid levels of hBD‐1 were: Hh = 229.52 ± 138.96 (median 199.26), Ph = 53.88 ± 58.17 (median 35.75), Pg = 57.11 ± 40.18 (median 39.90) and Pp = 55.31 ± 37.28 (median 54.19). No influence of site diagnosis (level 1; health/gingivitis/periodontitis) was observed; however, individual diagnosis (level 2; health/periodontitis) influenced the levels of hBD‐1 ( P < .001). Conclusion: Periodontally healthy individuals showed higher gingival crevicular fluid levels of hBD‐1 when compared to individuals with chronic periodontitis. This suggests a potential protective role of hBD‐1 in the susceptibility to chronic periodontitis.