Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes. (3rd April 2018)

• Record Type: Journal Article
• Authors: Jocken, Johan W. E.; Reijnders, Dorien; Canfora, Emanuel E.; Boekschoten, Mark V.; Plat, Joghum; Goossens, Gijs H.; Blaak, Ellen E.
• Is Part Of: Adipocyte. Volume 7:Number 2(2018)
• Page Start: 106
• Page End: 112
• Publication Date: 2018-04-03
• Subjects: Microbiota -- Lipolysis -- Fatty acid metabolism -- Adipose Tissue -- Obesity -- Insulin resistance; Fat cells -- Periodicals; Adipocytes -- Periodicals; 611.018276
• DOI: 10.1080/21623945.2018.1464366
• Languages: English
• ISSNs: 2162-3945

Abstract

ABSTRACT: The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and β-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective β-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue β-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.