STAT3 is required for proliferation and exhibits a cell type-specific binding preference in mouse female germline stem cells. Issue 2 (12th February 2018)

• Record Type: Journal Article
• Authors: Gu, Yunzhao; Wu, Jun; Yang, Wenxiao; Xia, Chao; Shi, Xinglong; Li, Hua; Sun, Jielin; Shao, Zhifeng; Wu, Ji; Zhao, Xiaodong
• Is Part Of: Molecular omics. Volume 14:Issue 2(2018)
• Page Start: 95
• Page End: 102
• Publication Date: 2018-02-12
• Subjects: Molecular biology -- Periodicals; Biochemistry -- Periodicals; Biological systems -- Periodicals; Molecular Biology; Computational Biology; Biochemistry; Biological systems; Molecular biology; Periodicals; Electronic journals; Periodicals; Fulltext; Internet Resources; Periodicals
• DOI: 10.1039/c7mo00084g
• Languages: English
• ISSNs: 2515-4184

Abstract

Abstract : LIF-mediated STAT3 signaling contributes to proliferation and exhibits a cell-specific binding pattern in mouse female germline stem cells. Abstract : LIF-mediated STAT3 signaling is critically involved in stem cells and development. However, its function in mouse female germline cells (FGSCs) remains elusive. In this study, we demonstrated that LIF-induced STAT3 activation contributes to the proliferation and undifferentiation maintenance of mouse FGSCs. Characterization of the STAT3-mediated transcriptional network by intersecting ChIP-seq and RNA-seq datasets revealed 405 direct target genes of STAT3, which are primarily involved in proliferation and germline development. In particular, we observed that STAT3 exhibits a FGSC-specific binding pattern when compared with mouse embryonic stem cells. Taken together, our study reported that the LIF-mediated STAT3 activation is actively involved in FGSCs and functions through a distinctive binding pattern across the FGSC genome. This cell-type specific binding preference provides an insight into understanding the genetic base for STAT3-driven cellular functions in germline stem cells.